HEMATOLOGICAL BIOMARKERS AND CYTOMORPHOLOGIC IN THE COMMON TOAD Rhinella arenarum

SALINAS, ZULMA ANAHÍ; BARAQUET, MARIANA; SALAS, NANCY E.; MARTINO, ADOLFO LUDOVICO

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: San Juan; Año: 2011 vol. 35 p. 235 - 235

0327-9545

The differential amount of white blood cells and cytomorphometry were studied in R. arenarum as biomarkers of genotoxicity in environments with different degrees of impact. The study was conducted in the pond Las Brujas (UNRC) and the urban lake Villa Dalcar, Rio Cuarto, Córdoba. Ten individuals were captured per site and took samples of blood by puncture of angularis vein. The smears were stained with May-Grünwald-Giemsa and observed under a microscope. The total number of white blood cells for the pond Las Brujas was: 382.7 ± 162.7 / 10000 erythrocytes and 364 ± 312.9/ 10000 erythrocytes for Villa Dalcar. The ratio N/L (neutrophils/ lymphocytes) was 0.17 for the pond Las Brujas and 0.24 for Lake Villa Dalcar. No significant differences were observed between sites for the variables for the hemogram (U test, p> 0.05), except for monocytes (p = 0.002). For the blood morphometry, no significant differences between sites (U test, p> 0.05) were found. The ratio N/ L is similar to other research in R. arenarum. The results of this study indicate that hematological evaluation allows for early detection of physiological changes related to processes of environmental pollution