Hematological biomarkers of environmental impact in urban and rural environments of Córdoba province.

SALINAS ZULMS ANAHÍ ; BARAQUET MARIANA; SALAS, NANCY E.; MARTINO, ADOLFO LUDOVICO

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 118 - 118

0327-9545

The use of hematological biomarkers allows to detect observable or measurable changes in the exposure of organisms to xenobiotics. In this work, differential count and cytomorphometry of white blood cells are proposed as biomarkers of genotoxicity in Bufo arenarum inhabiting in environments with different alteration degrees of Río Cuarto (Villa Dalcar and UNRC), rural areas and a control site. The blood samples were obtained by angularis vein puncture of 93 individuals and the smears were stained with May Grunwal-Giemsa and observed by using a microscope. The mean leukocyte of Villa Dalcar was higher compared to the other sites (425.48 ± 481.34). Leukocyte formula showed significant differences between Villa Dalcar and control site respect to monocytes and basophils (W=62.5, p=0.000; W=103.5, p=0.007), with higher values in the first site. Cytomorphometry also showed significant differences between Villa Dalcar and control site for both cell a nuclear area (p