C-BANDING PATTERNS AND MEIOTIC BEHAVIOR IN HYPSIBOAS PULCHELLUS AND H. CORDOBAE (ANURA, HYLIDAE)

BARAQUET MARIANA; SALAS NANCY EDITH; MARTINO ADOLFO LUDOVICO

Journal of Basic and Applied Genetics

Sociedad Argentina de Genética

Año: 2013 vol. 24 p. 32 - 39

1852-6233

In this work, we observed and evaluated C-banding patterns and meiotic behaviour of Hypsiboas pulchellus and H. cordobae. Twenty metaphasic cells per individual were analyzed, which were obtained from intestinal and testis cells using conventional and C-banding techniques. The two species presented 2n= 24 chromosomes. Pairs 1 and 8 to 12 were metacentric whereas pairs 2 to 7 were submetacentric. The centromeric region of all chromosomes was positively C-banded but the C-banding pattern varied between species. At meiosis, cells with 12 bivalents were observed. Bivalents usually presented two terminal chiasmata, although the largest pair presented only one terminal chiasma. The cytogenetic data reconfirms the diploid somatic chromosome number of H. pulchellus and H. cordobae, species that exhibited identical chromosome morphology. The remarkable similarities between the two species are an indication of the close relationship among members of the H. pulchellus group; however C-banding patterns of Hypsiboas species are distinct and may be also referred to as species-specific. Meiotic behavior is quite similar to the behaviour of most frogs, which possess only one or two -usually terminal- chiasmata, resulting in the typical linear or ring appearance of bivalents.