INVESTIGADORES
OKLANDER Luciana Ines
artículos
Título:
Preservation and Extraction of DNA from feces in howler monkeys (Alouatta caraya).
Autor/es:
L. OKLANDER, M. MARINO, G. ZUNINO, D. CORACH
Revista:
NEOTROPICAL PRIMATES
Editorial:
IUCN/SSC
Referencias:
Lugar: Washington; Año: 2004 vol. 12 p. 59 - 63
ISSN:
1413-4705
Resumen:
In this study we evaluate the effectiveness of four preservation methods and three extraction methods for recovering DNA from fecal samples of a New World primate, the black and gold howler monkey Alouatta caraya. Our assay of the effectiveness of each of these methods is the consistent amplification of a microsatellite or STR locus Ap-74 originally isolated from a different species of howler monkey and known to be present in A. caraya. Our results indicate that the best and least expensive method to preserve faecal samples seems to be in solid salt (NaCl), and the silica extraction method was the only one that allowed us to obtain results. This contribution offers a detailed procedure for preservation and extraction of DNA from non invasive samples that is suitable for use on wholly arboreal primates.
