Allopregnanolone modulates striatal dopaminergic activity of rats under different gonadal hormones conditions

M.R.LACONI; P.C.REGGIANI; A. PENISSI; R. YUNES; CABRERA R.

NEUROLOGICAL RESEARCH.

W.S.Maney and Son Ltd

Lugar: USA; Año: 2007 vol. 29 p. 622 - 627

0161-6412

Objectives: Progesterone modulates dopamine (DA) release in corpus striatum. Our objective was to evaluate the effect of the i.c.v injection of the neurosteroid allopregnanolone (ALL), a progesterone metabolite on dopaminergic activity in the corpus striatum of rats under different gonadal hormonal conditions. was to evaluate the effect of the i.c.v injection of the neurosteroid allopregnanolone (ALL), a progesterone metabolite on dopaminergic activity in the corpus striatum of rats under different gonadal hormonal conditions. was to evaluate the effect of the i.c.v injection of the neurosteroid allopregnanolone (ALL), a progesterone metabolite on dopaminergic activity in the corpus striatum of rats under different gonadal hormonal conditions. Progesterone modulates dopamine (DA) release in corpus striatum. Our objective was to evaluate the effect of the i.c.v injection of the neurosteroid allopregnanolone (ALL), a progesterone metabolite on dopaminergic activity in the corpus striatum of rats under different gonadal hormonal conditions. Methods: We have measured the concentrations of DOPA, DA and DOPAC (main metabolite of DA) in the corpus striatum in estrus and diestrus rats and in ovariectomized rats without hormonal replacement (OVX group) and primed with estrogen and progesterone (OVXi DA) in the corpus striatum in estrus and diestrus rats and in ovariectomized rats without hormonal replacement (OVX group) and primed with estrogen and progesterone (OVXi DA) in the corpus striatum in estrus and diestrus rats and in ovariectomized rats without hormonal replacement (OVX group) and primed with estrogen and progesterone (OVXi We have measured the concentrations of DOPA, DA and DOPAC (main metabolite of DA) in the corpus striatum in estrus and diestrus rats and in ovariectomized rats without hormonal replacement (OVX group) and primed with estrogen and progesterone (OVXii group). Additionally, we have used the aromatic acid decarboxylase inhibitor NSD in order to evaluate the function of tyrosine hydroxylase (TH), the rate-limiting enzyme of dopamine synthesis. Results: ALL significantly decreased the striatal concentrations of both DA and DOPAC in the estrus. On the other hand, ALL increased significantly the levels of DA in the OVXi group. The DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. estrus. On the other hand, ALL increased significantly the levels of DA in the OVXi group. The DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. estrus. On the other hand, ALL increased significantly the levels of DA in the OVXi group. The DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. ALL significantly decreased the striatal concentrations of both DA and DOPAC in the estrus. On the other hand, ALL increased significantly the levels of DA in the OVXi group. The DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. i group. The DOPA accumulation in OVXi after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. i after NSD treatment in the ALL-treated groups was greater than in the vehicle group. However, the estrus group did not modify the DOPA accumulation after NSD injection. Discussion: Our results suggest that ALL could modulate the dopaminergic transmission in the corpus striatum by causing changes in the activity of TH and/or in the pre- and post-synaptic dopaminergic terminals in the corpus striatum. This neurosteroidal mechanism could be a new kind of neurotransmitter systems modulation accomplished on TH activity itself and/or on the second messengers not related to ionic channels. Additionally, our results reinforce the idea of a close relationship between the fast non-genomic mechanism of ALL and t corpus striatum by causing changes in the activity of TH and/or in the pre- and post-synaptic dopaminergic terminals in the corpus striatum. This neurosteroidal mechanism could be a new kind of neurotransmitter systems modulation accomplished on TH activity itself and/or on the second messengers not related to ionic channels. Additionally, our results reinforce the idea of a close relationship between the fast non-genomic mechanism of ALL and t corpus striatum by causing changes in the activity of TH and/or in the pre- and post-synaptic dopaminergic terminals in the corpus striatum. This neurosteroidal mechanism could be a new kind of neurotransmitter systems modulation accomplished on TH activity itself and/or on the second messengers not related to ionic channels. Additionally, our results reinforce the idea of a close relationship between the fast non-genomic mechanism of ALL and t Our results suggest that ALL could modulate the dopaminergic transmission in the corpus striatum by causing changes in the activity of TH and/or in the pre- and post-synaptic dopaminergic terminals in the corpus striatum. This neurosteroidal mechanism could be a new kind of neurotransmitter systems modulation accomplished on TH activity itself and/or on the second messengers not related to ionic channels. Additionally, our results reinforce the idea of a close relationship between the fast non-genomic mechanism of ALL and t