Nanodevices for the immobilization of therapeutic enzymes

V.E. BOSIO; G.A. ISLAN; Y. N. MARTINEZ; N. DURAN; G.R. CASTRO

CRITICAL REVIEWS IN BIOTECHNOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2016 vol. 36 p. 447 - 464

0738-8551

Therapeutic enzymes are one of the most promising applications of this century in the field ofpharmaceutics. Biocatalyst properties can be improved by enzyme immobilization on nanoobjects,thereby increasing stability and reusability and also enhancing the targeting to specifictissues and cells. Therapeutic biocatalyst?nanodevice complexes will provide new tools for thediagnosis and treatment of old and newly emerging pathologies. Among the advantages of thisapproach are the wide span and diverse range of possible materials and biocatalysts thatpromise to make the matrix?enzyme combination a unique modality for therapeutic delivery.This review focuses on the most significant techniques and nanomaterials used for enzymeimmobilization such as metallic superparamagnetic, silica, and polymeric and single-enzymenanoparticles. Finally, a review of the application of these nanodevices to different pathologiesand modes of administration is presented. In short, since therapeutic enzymes constitute ahighly promising alternative for treating a variety of pathologies more effectively, this review isaimed at providing the comprehensive summary needed to understand and improve thisburgeoning area.