Alginate Lyase and Ciprofloxacin co-immobilization on biopolymeric microspheres for Cystic Fibrosis treatment

GERMAN A. ISLAN; VALERIA E. BOSIO; GUILLERMO R. CASTRO

MACROMOLECULAR BIOSCIENCE

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 p. 1 - 4

1616-5187

A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.