Microcystis aeruginos strain [D-Leu1] Mcyst-LR producer, from Buenos Aires province, Argentina

ROSSO, L.; SEDAN, D.; KOLMAN, M.; CAIXACH, J.; FLORES, C.; OTEIZA, J. M.; SALERNO, G.; ECHENIQUE, R.; GIANNUZZI, L.; ANDRINOLO, D.

Journal of Coastal Life Medicine

Editorial Board of Journal of Coastal Life Medicine

Año: 2014 p. 287 - 296

2309-5288

Objective. In this study, we show the Toxicological and phylogenetic characterization of a native Microcystis aeruginosa strain (named CAAT 2005-3) isolated from a water body of Buenos Aires Province, Argentine. Methods. A Microcistis aeruginosa strain was isolated from the drainage canal of the sewage treatment in the town of Pila, Buenos Aires Province, Argentina and acclimated to laboratory conditions. The amplification of cpcBA-IGS Phcocyanin (PC, intergenic spacer and flanking regions) was carried out in order to build phylogenetic tree. An Exactive /Orbitrap mass spectrometer equipped with an electrospray ionization (ESI) source (Thermo Fisher Scientific, Bremen, Germany) were used for the LC/ESI-HRMS microcystins analysis. The number of cell.mL-1 and [D-Leu1] Mcyst-LR production obtained as a function of time was modelled using Gompertz equation Results. The phylogenetic analysis showed that the sequence clustered with others M. aeruginosa sequences obtained from NCBI . The first Argentinian strain of M. aeruginosa (CAAT 2005-3) growing under culture conditions maintain the typical colonial architecture of M. aeruginosa with profuse mucilage. M. aeruginosa CAAT2005-3 expresses a toxin variant, that was identified by LC-HRMS / Orbitrapas as [D-Leu1] microcystin-LR ([M + H] + = 1037.8 m / z). Conclusions. [D-Leu1] microcystin-LR has been also detected in M. aeruginosa samples from Canada, Brasil and Argentina. This work provides the basis for technological development and production of analytical standards of toxins present in our region.