ABOUT PESTICIDES AND OTHER DEMONS: INDUCTION OF TUMOR ANGIOGENESIS IN OUR BREASTS

ZÁRATE LV ; PONTILLO CA ; ESPAÑOL A ; MIRET N ; CHIAPPINI F; COCCA C; ÁLVAREZ L; KLEIMAN DE PISAREV DL; SALES ME; RANDI AS

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2018

Breast cancer is themost frequent tumor in women worldwide. Pesticides that act as endocrinedisruptors have been shown to affect normal mammary development. In this study,we examined the action of Hexachlorobenzene (HCB) and Chlorpyrifos (CPF) on angiogenesis in mammary carcinogenesis in vivo and in vitro. We analyzed the levels of proangiogenic factors such asvascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2), as wellas the expression of the nitric oxide synthases (NOS) by WB and theirproduction of nitric oxide (NO) by Griess reagent. In a xenograft model withMCF-7 cells (+ERα), HCB (3 mg/kg b.w.) and CPF(0.1 mg/kg b.w.) enhance angiogenic switch (number of vessels/mm2)and increase VEGF expression in mice skin (p