THE EFFECT "IN VIVO" OF THE ANTHELMINTIC TRICLABENDAZOLE ON FASCIOLA HEPATICA.

SCARCELLA, S.; FIEL, C; SAUMELL, C; GENTILE, M. L.; FELIPE, A.; ALZOLA, R.; SOLANA, H.

Buenos Aires

Congreso; XXXIX Reunion Anual de la Asociación Argentina de Farmacología Experimental; 2007

Sociedad argentina de farmacologia experimental

The efficacy test are the main tools for the characterization of drug and the test recommended for the characterization of anthelmintics. The parasite, Fasciola hepatica, has a worldwide distribution and infects a wide variety of mammalian hosts, including the human. Triclabendazole (TCBZ) is an antihelmintic benzimidazole widely used to control the fluke F. hepatica.  In this work were evaluated through histopatological and immuno histochemical techniques the alterations generated in F. hepatica exposed "in vivo" at TCBZ. Four healthy bovines without antecedents of fasciolasis was inoculated with 400 metacercarias each one. Two of the inoculated bovines (experimental) were treated to the 90 days post infection with 8 mg/Kg. of TCBZ and sacrificed jointly with the controls to day 105. The flukes of the bovine controls showed testicular tubes full of cells in different states from the espermatogenesis with abundant viteline cells and uterus with mature eggs. The fluke exposed to TCBZ displayed their testes with abundant peripheral vacuolización, significant diminution of the number of viteline cells and uterus without detection of egg. These findings support the fact that despite the survival of the trematode to the drug exposure, they showed a marked infertility, which would impede them to continue their biological cycle. These findings induced us to review the validity of the controlled efficacy test, which is based only on the counting of live trematodes at the moment of host sacrifice.