Coautor del trabajo titulado - A Maternal Diet Enriched In Extra Virgin Olive Oil Does Not Prevent Reduced Uteri Weight and Decidual PPAR Levels in The Offspring of Diabetic Rats

CINTIA ROMINA GATTI*, SABRINA LORENA ROBERTI*, DALMIRO GOMEZ RIBOT, ROMINA HIGA, ALICIA JAWERBAUM

MODO VIRTUAL

Congreso; LATIN AMERICAN DOHaD 2020. One World One Health. On the Web.; 2020

Background andAim:Maternaldiabetes increases the risks of maternal, fetal and placental complications andleads to the programming of metabolic and cardiovascular diseases in theoffspring. These alterations are in part related to a maternal diabetes-inducedprooxidant-proinflammatory intrauterine environment, which may alter the uterusin the female offspring. Our recent studies have shown that a dietarysupplementation with extra virgin olive oil (EVOO) is able to reduce markers ofthe prooxidant-proinflammatory state in the placenta and different fetal organsin experimental models of diabetes and pregnancy. EVOO is highly enriched inoleic acid, a monounsaturated fatty acid that activates Peroxisome ProliferatorActivated Receptors (PPARs). PPARs are involved in decidualization andanti-inflammatory processes. The aim of this work was to evaluate the uterus ofthe offspring of diabetic rats that receive or not an EVOO enriched diet duringpregnancy, evaluating the weight of decidualized and not-decidualized uteri ata prepubertal age, as well as addressing PPARs expression in the decidua at apostimplantation stage.   Methods:A mildpregestational diabetic rat model was induced in F0 females by neonataladministration of streptozotocin (90 mg/kg sc). Control and diabetic femaleswere mated with healthy males and were fed or not with a 6% olive oil enricheddiet from day 1 of pregnancy until parturition. The offspring were fed astandard diet and the uteri of the female offspring (F1) were evaluated a) at aprepuberal stage: 30 day postnatal, with or without induction ofdecidualization with PMSG (50 UI) and hCG (50 UI) and b) on day 9 of pregnancy.At the prepuberal stage non-decidualized and decidualized uteri were weighted,and on day 9 of pregnancy, the expression of PPARalpha, PPARgamma and PPARdeltawas evaluated in the decidua.  Results:At a prepubertal stage, the offspring of diabetic rats(Diabetic Group) showed a reduction in weight in the non-decidualized uteri(36%, p