Decidual abnormalities in the prepubertal period and at day 9 of pregnancy in diabetic rat offspring.

CINTIA ROMINA GATTI*, SABRINA LORENA ROBERTI*, EVANGELINA CAPOBIANCO, DALMIRO GÓMEZ RIBOT, ALICIA JAWERBAUM

Simposio; III Simposio Colombiano de placenta e Interacción materno-fetal 2020; 2020

Introduction:Maternal diabetes induces an intrauterine prooxidant/proinflammatoryenvironment, associated to impaired PPAR pathways, which could alter theoffspring?s uterus.  Objectives:To evaluate, in control and diabetic rat offspring, the uterine weight(decidualized or not) in the prepubertal period, and decidual PPARs proteinexpression on day 9 of pregnancy.  Methods:Diabetes was induced in F0 rats by neonatal administration of streptozotocin(90 mg/kg sc). Females were mated with healthy males to study the offspring?suterus a) on postnatal day 30, with or without induction of decidualization(PMSG/hCG, 50 IU) and b) on day 9 of pregnancy. Uteri were weighed at theprepubertal stage and decidual PPAR levels were evaluated on day 9 of pregnancy. Results:In the prepubertal stage, the uterus of the offspring of diabetic rats showedreduced weight (p<0.05 vs Control), and the induction with PMSG/hCGincreased its weight to values similar to controls. In pregnancy, number ofimplantations and resorptions did not change in the studied groups. Proteinexpression of PPARalpha, PPARgamma and PPARdelta were found reduced (p<0.05vs Control).  Conclusions:Maternal diabetes alters the offspring?s uterus at prepuberty and leads toreduced expression of decidual PPARs in pregnancy, possibly contributing to anF1 prooxidant/proinflammatory intrauterine environment.