Experimental gestational diabetes induces a pro-inflammatory and pro-oxidant environment in maternal and fetal livers

DAIANA DEBORA FORNES, MARÍA FLORENCIA HEINECKE, EVANGELINA CAPOBIANCO, ALICIA JAWERBAUM

Congreso; International Federation of Placenta Associations; 2019

Objectives: Gestational diabetes (GDM) is a prevalent disease associated with a pro-oxidant and pro-inflammatory environment and with adverse consequences to both the mother and the offspring. The fetal liver is highly exposed to metabolic abnormalities, potentially leading to alterations in its development, which would affect the metabolic function in adulthood. The aim of this work was to evaluate whether pro-inflammatory and pro-oxidant markers are increased in maternal and fetal livers in GDM rats.Methods: GDM was spontaneously induced by intrauterine programming in the offspring (F1) of diabetic rats (F0 diabetic rats were obtained through neonatal streptozotocin administration). In control and GDM rats, maternal and fetal livers were explanted on day 21 of gestation for further evaluation of pro-oxidant and pro-inflammatory markers (nitric oxide production (nitrates/nitrites measurement), lipoperoxidation (TBARS measurement), and the protein expression of metalloproteinase-2 (MMP2), connective tissue growth factor (CTGF), and Manganese Superoxide Dismutase (Mn-SOD)) as well as the levels of PGC-1alpha, a PPAR co-activator that regulates pro-oxidant/pro-inflammatory processes. Results: In the maternal liver of rats with GDM the levels of nitrates/nitrites were increased (254%, p