Advanced maternal age affects senescence and matrix metalloproteinase 2 in maternal-embryo interface during placenta formation.

MARÍA LAURA LEONARDI, LAUTARO RECCHIA, CINTIA ROMINA GATTI, ALICIA JAWERBAUM, ROMINA HIGA

Congreso; V Meeting of the Latin American Regional Society for Developmental Origins of Health and Disease (LA-DOHaD).; 2023

Background: Pregnancy at Advanced Maternal Age (~35 years, AMA) induces obstetric complications and neonatal adverse outcomes1,2. During early pregnancy, an optimal decidual function is essential for a correct placenta and embryo development3. MMP2, a protease of senescence-associated secretory phenotype (SASP), have an important role in tissue remodeling during trophoblast invasion and embryo morphogenesis4. We have previously observed that decidua of a rat AMA model showed increased MMP2 and embryonic growth impairments. Here, our aim was to evaluate in the decidua from AMA rats, the localization of 4HNE (marker of lipoperoxidation) and mRNA levels of p21 (marker of senescence) during placenta formation. Also, to evaluate mRNA levels of p21 and MMP2 in the placenta and the embryo from AMA rats together with embryonic ROS levels. Methods: 3 months old (Control) and 10 months old (AMA) Wistar rats were mated with young males. Decidua, placenta and embryos were obtained on day 12 of pregnancy. mRNA levels of p21 and Mmp2 were measured by RT-qPCR (n=8), 4-HNE by immunohistochemistry (n=5) and ROS by DCF-DA probe (n=7).Results: The decidua of AMA showed increased mRNA levels of p21 (+54%, p