Impaired microRNA-21 and prolactin receptor levels in the decidua of the prepubertal offspring of diabetic rats are prevented by maternal diets enriched in olive oil.

CINTA ROMINA GATTI, DALMIRO GOMEZ RIBOT, ROMINA HIGA, ALICIA JAWERBAUM.

Congreso; IFPA 2023; 2023

Impaired microRNA 21 and prolactin receptor levels in the decidua of the prepuberal offspring of diabetic rats are prevented by maternal diets enriched in olive oil. OBJECTIVESDiabetes mellitus is a metabolic pathology that programs offspring´s diseases. We previously found reduced prolactin levels in the decidua of the offspring of diabetic rats, an alteration prevented by a maternal diet enriched in olive oil. MicroRNA 21 (miR-21) is a microRNA that regulates cell proliferation and decidualization and PPARdelta a PPAR isotype relevant in decidualization. Here, we aim to evaluate the levels of miR-21, PPARδ and the prolactin receptor PRLR in the decidualized uteri of prepuberal offspring of diabetic rats fed or not with an olive oil-enriched diet.METHODSA mild pregestational diabetic rat model was induced in F0 females by neonatal administration of streptozotocin (90 mg/kg sc). Control and diabetic females were mated with healthy males and received a 6% olive oil-enriched diet or a standard diet from day 1 of pregnancy until parturition. The offspring were fed a standard diet and the uteri of the female offspring (F1) were evaluated on postnatal day 30, after induction of decidualization with PMSG (50 UI) and hCG (50 UI). MiR-21, was evaluated by RT-qPCR and PPARδ and PRLR were evaluated by Western Blot. RESULTSThe offspring of diabetic rats showed a reduction in miR-21 levels in the decidualized uteri (0.24 fold-change, p