• Fetal programming of altered decidualization in the offspring of diabetic rats.

CINTIA ROMINA GATTI, SABRINA LORENA ROBERTI, LAUTARO RECCHIA, ROMINA HIGA, ALICIA JAWERBAUM

Congreso; Reunión anual de Sociedades Biocientíficas (SAIC 2022); 2022

Introduction: Diabetes mellitus is a metabolic pathology that leads to placental, fetal and offspring alterations. Little is known regarding putative alterations in the uteri of the offspring of diabetic rats, and if maternal diets enriched in olive oil can prevent these alterations. Aim: To evaluate the expression of prolactin (PRL) and fatty acid-binding protein 4 (FABP4), key proteins in decidualization, and 4-Hydroxynonenal (4-HNE) levels, a pro-oxidant marker, in the decidualized uteri of prepubertal diabetic rat offspring fed or not with an olive oil-enriched diet.Methods: Mild pregestational diabetic rats were obtained by neonatal administration of streptozotocin (90 mg/kg sc). Control and diabetic rats received a 6% olive oil-enriched diet or a standard diet from day 1 of pregnancy until parturition. The offspring were fed a standard diet and the uteri of the offspring (F1) evaluated at 30 days postnatal, after decidualization induction with PMSG (50 UI) and hCG (50 UI). Prl1 and Fabp4 were evaluated by RT-qPCR, PRL and FABP4 levels by Western blot and 4-HNE by immunohistochemistry. Results: The decidualized uteri of diabetic rat offspring showed reduced Prl1 mRNA levels (0.38 fold-change) and PRL protein levels (47%) compared to controls (p