• Uterine prolactin and IGFBP1 in the offspring of diabetic rats: Effects of a maternal diet enriched in olive oil.

CINTIA ROMINA GATTI; SABINA LORENA ROBERTI; MARÍA FLORENCIA HEINECKE, ROMINA HIGA, ALICIA JAWERBAUM.

Congreso; VIII Congreso de la Asociación Latinoamericana de Interacción Materno Fetal y Placenta (SLIMP); 2022

SLIMP

Objectives: Diabetes mellitus is a metabolic pathology that leads to placental, fetal, and offspring alterations. Little is known regarding putative alterations in the uteri of the offspring of diabetic rats, and whether maternal diets enriched in olive oil, can prevent these alterations. Both prolactin and insulin growth factor binding protein 1, respectively codified by Prl1 and Igfbp1 genes, are endocrine mediators of decidualization. Here, we aim to evaluate Prl1 and Igfbp1 gene expression in the decidualized uteri of prepuberal offspring of diabetic rats fed or not with an olive oil-enriched diet. Methods: A mild pregestational diabetic rat model was induced in F0 females by neonatal administration of streptozotocin (90 mg/kg sc). Control and diabetic females were mated with healthy males and received a 6% olive oil-enriched diet or a standard diet from day 1 of pregnancy until parturition. The offspring were fed a standard diet and the uteri of the female offspring (F1) were evaluated at 30 days postnatal, after decidualization induction with PMSG (50 UI) and hCG (50 UI). Uterine Prl1 and Igfbp1 mRNA levels were evaluated by RTqPCR. Results: The offspring of diabetic rats showed a reduction in Prl1 mRNA levels in the decidualized uteri (0.38 fold-change, p