INVESTIGADORES
CANOSA Luis Fabian
congresos y reuniones científicas
Título:
DETECTION OF ALTERNATIVE SPLICING EVENTS IN THE KISSPEPTIN RECEPTORS IN PEJERREY (Odontesthes bonariensis)
Autor/es:
MECHALY AS; TOVAR O; CAMPANELLA D; CANOSA LF; ORTI G; SOMOZA GM
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
In mammals, the kisspeptin system is composed by the
kisspeptin neuropeptide ligand and its associated receptor, KISSR. This system is primarily involved
in the neuroendocrine regulation of puberty and sexual maturation. Recent
studies have demonstrated the existence of a teleost-specific whole genomic
duplication. Then, several fish species presented two kissr genes (Kiss1rb and Kiss2rb). In this study, we report the
presence of two kisspeptin receptors in the pejerrey, Odontesthes bonariensis: pjKiss1rb
and pjKiss2rb. The genomic structure
of pjKiss1rb comprises seven exons
and six introns. On the other hand pjKiss2b
comprises five exons and four
introns. In the case of pjKiss2rb, two distinct transcripts were
detected having a difference of 100 bp in length. The longer transcript contained
an insert of 98 nucleotides caused by the retention of intron III. The predicted aminoacid sequence of this
insert comprised 32 aminoacids with two premature stop codons. Future studies
will aim to understand if this putative truncate protein may lead to a non-functional
receptor and to our understanding of the physiological functions of the
kisspeptin signaling system.