Mineralización del tertadeciltrimetilamonio utilizando Pseudomonas putida A

LÓPEZ, FG; LIFFOURRENA, AS; LUCCHESI, GI

Villa General Belgrano, Córdoba

Jornada; XIV Jornadas Científicas de la Asociación de Biología de Córdoba; 2003

Sociedad de Biología de Córdoba

CompletE mineralizaTion OF tetradecyl trimethylammonium uSinG P.  putida A. López, F.G., Liffourrena, A.S. and Lucchesi, G.I. Dpto. Biología Molecular. FCEFQyN. UNRC. Río Cuarto. Córdoba. E-mail: glucchesi@exa.unrc.edu.ar Quaternary ammonium compounds (QAC’s) are commercial chemicals commonly used in a variety of products. Considering the resistance to biological degradation and the complete mineralization of QAC’s achieves with consortia of at least two microorganisms; the purpose of this study was to evaluate the pure culture potential for the complete degradation of a QAC’s, tetradecyltrimethylammonium (TDTMA). P. putida A grew in liquid HPi-BSM medium containing 50mg/l of TDTMA as the sole C, N or C and N source. The growth occurred concomitantly with TDTMA disappearance and the level consumed at 48 h was 28mg/l. At 72 h the TDTMA consumption increased a 10% but the growth was not significant. The increase in cell protein when the culture reached the stationary phase was 11.4 mg/l. This value represented about 86% the initial N-TDTMA incorporated into the cell biomass. When cells grew on glucose plus TDTMA, analysis of supernatants showed that it was fully consumed in 96h. The increase in cell protein was 18.8 mg/l and 97% the N-TDTMA was incorporated into cell biomass. A monooxygenase activity was measured and implicated in the TDTMA degradation initial steps. This enzyme activity was present in cell-free extracts of cells grown on TDTMA as the C, N or C and N source but not in cells grown on glucose and NH4Cl showing that the TDTMA oxidation is dependent on inducible systems. Taking into account that others bacteria can’t grow on different QAC’s as a sole C and N source, this strain is fairly suitable for biodegradation studies.