GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes

GUERRA LILIANA; SUÁREZ CECILIA; SOTO DANIELA; SCHIAPPACASSE AGUSTINA; SAPOCHNIK DAIANA; SACCA PAULA; PIWIEN-PILIPUK GRACIELA; PERAL BELEN; CALVO JUAN CARLOS

CLINICAL & TRANSLATIONAL ONCOLOGY

SPRINGER

Lugar: Barcelona; Año: 2015

1699-048X

Introduction. In the mammary gland, the involution thatoccurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal?epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124?131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods. Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.Results. We described, for the first time, that breast epi-thelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPb expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.Conclusions. Taken together, these results suggest thatGAL3ST2 would interfere with normal differentiation of3T3-L1 preadipocytes; raising the possibility that it mayaffect normal differentiation of stromal preadipocytes andbe a link to tumor metastatic capacity.