Angiogenesis and VEGF-A pathway are involved in the mechanism by which INGAP_PP increases B-cell mass and function

ROMAN CL; MAIZTEGUI B; FLORES LE; DEL ZOTTO HH; GAGLIARDINO JJ

Buenos Aires

Congreso; 21st IAP Meeting pancreas 2017; 2017

International Association of Pancreatology

Background:Islet angiogenesis is needed for embryonic B-cell differentiation and INGAP-PP administration increases Beta-cell mass and function in rats.Aim:To determine the role of islet angiogenesis in the mechanism by which INGAP-PP enhances B-cell mass and function. Methods: In vivo: adult Wistar rats were treated (ip) for 10 days with saline (Control) or INGAP-PP (500µg/day). Thereafter, serum parameters levels were measured and HOMA-IR and ? B were calculated. Cell apoptotic rate and gene expression (qPCR and western blot) of angiogenesis and apoptotic markers were determined. In vitro: islets isolated from normal rats were cultured for 4 days in different composition: Control; INGAP-PP (10µg/ml); Rapamycin (10ng/ml + control or INGAP-PP); and VEGF (10ng/ml). We determined VEGF released to the medium (ELISA) and glucose-stimulated insulin secretion (RIA) Statistics: ANOVA and t test; significant differences were considered when p