Dietar fatty acids and unsaturated fatty acids on triacylglycerides metabolism in mice

SAÍN J; GONZÁLEZ MA; LAVANDERA, JV; SCALERANDI MV; VES LOSADA, A; BERNAL CA

Mar del Plata

Conferencia; LI Reunión Anual de SAIB; 2015

SAIB

The amount and type of dietary fat could modulate the levels of triacylglycerol (TAG) in plasma and tissues. The aim was to investigate the effects of trans fatty acids (TFA) on TAG regulation in mice fed different unsaturated fatty acid (UFA) diets. Male CF1 mice (22 g) were fed (30 d) with a standard diets differing in dietary n-3/n-6/n-9 UFA ratios: Canola (C:1/2/6), Maize (M:1/53/31) or Olive (O:1/10/76) oils containing 0.75% of TFA (Ct, Mt and Ot, respectively) or not. Expression and activities of lipogenic and oxidative enzymes, serum and liver TAG content, hepatic TAG secretion rate (TGSR), and adipose tissue (AT) and muscle lipoprotein lipase (LPL) activities, were assessed. TFA induced hepatic TAG accretion in Ot and Ct groups (100%) vs. their respective control group, being associated with an exacerbated expression (FAS 300%; ACC 100%) and activity (FAS 270%; ACC 140%) of lipogenic enzymes induced by the increased SREBP1a expression (800%) in Ot group. In Ct, it was related to a lower CPT-I activity (-350%). These effects, lead to an enhanced TGSR in Ot (260%) and Ct (160%) groups vs. their controls, however the LPL activity in AT (200% in Ot and Ct vs. O and C, respectively) normalize the levels of serum TAG. In Mt group no changes were observed in these parameters. In conclusion, TFA altered TAG metabolism by different mechanisms depending on the type of dietary UFA.