INVESTIGADORES
CAVALLO Pablo Cesar
artículos
Título:
Cysteine modified polyaniline films improve biocompatibility for two cell lines
Autor/es:
EDITH I. YSLAS; CAVALLO PABLO C; ACEVEDO DIEGO F; BARBERO CESAR A; VIVIANA A. RIVAROLA
Revista:
MATERIALS SCIENCE & ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 51 p. 51 - 56
ISSN:
0928-4931
Resumen:
This work focuses on one of the most exciting application areas of
conjugated conducting polymers, which is cell culture and tissue
engineering. To improve the biocompatibility of conducting polymers we
present an easy method that involves the modification of the polymer
backbone using l-cysteine. In this
publication, we show the synthesis of polyaniline (PANI) films supported
onto Polyethylene terephthalate (PET) films, and modified using
cysteine (PANI-Cys) in order to generate a biocompatible substrate for
cell culture. The PANI-Cys films are characterized by Fourier Transform
infrared and UV?visible spectroscopy. The changes in the hydrophilicity
of the polymer films after and before the modification were tested using
contact angle measurements. After modification the contact angle
changes from 86° ± 1 to 90° ± 1, suggesting a more hydrophylic surface.
The adhesion properties of LM2 and HaCaT cell lines on the surface of
PANI-Cys films in comparison with tissue culture plastic (TCP) are
studied. The PANI-Cys film shows better biocompatibility than PANI film
for both cell lines. The cell morphologies on the TCP and PANI-Cys film
were examined by florescence and Atomic Force Microscopy (AFM).
Microscopic observations show normal cellular behavior when PANI-Cys is
used as a substrate of both cell lines (HaCaT and LM2) as when they are
cultured on TCP. The ability of these PANI-Cys films to support cell
attachment and growth indicates their potential use as biocompatible
surfaces and in tissue engineering.