EFFECT ON SPERM QUALITY OF DIFFERENT CRYOPROTECTANS IN SPERM OF Chinchilla lanigera

M.C.GRAMAJO BÜHLER; F.J. PUCCI ALCAIDE; G.SÁNCHEZ TORANZO

ZYGOTE

CAMBRIDGE UNIV PRESS

Lugar: cambridge; Año: 2012 p. 1 - 7

0967-1994

Chinchilla lanigera is an endangered species and the development of cryopreservation protocols of its gametes is a useful tool in the application of assisted reproduction techniques. The study of the functionality of the spermatozoa punctured from the cauda epididymis was performed with fresh and frozen/thawed samples with three cryoprotective media (Test Yolk Buffer, Sucrose and Glycerol), analyzing the effect that these media had on sperm physiology during the freezing, storage and later thawing process. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation being also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to cryopreserve epididymal sperm in this species is Test Yolk Buffer. This medium was the one that least affected the above physiological parameters, especially at the level of the genetic material.