Study of the optimal run condition for a chomatographic porous column.

MIRNA SANCHEZ; MARCELO FERNANDEZ LAHORE; MARIANO GRASSELLI

Córdoba

Congreso; Sab2013; 2013

Study of theoptimal run condition for a chomatographic porous column.MirnaL. Sanchez  [1,2],MarceloFernandez-Lahore [2] and Mariano Grasselli[1]      misanchez@jacobs-university.de[1]Universidad Nacional de Quilmes - IMBICE (CONICET). [2]Jacobs University Bremen.               Modern biotechnology heavily depends on theavailability of efficient processes, which have to generate competitiveproducts in terms of quality and cost. Most commonly, thepurification of bioproducts is based on chromatographic platforms. However, the low productivity imposed by diffusionallimitations, the need for multiple separation steps, and the requirement ofextensive solids separation, drives to a technological situation characterisedby low global bioproduct yields.1In a previous work, we fabricated porous, opened, andreticulated polyurethane foams supporting a functional hydrogel layer. Preliminarystudies showed that the adsorptive properties of these materials allow thereversibly binding of proteins by ion exchange mechanisms.Dynamic bindingcapacity tests (DBC) were performed employing lysozyme (HEWL) on a cationexchanger matrix. DBC experiments were carried out as a function column length,compression degree and flow velocity. Furthermore, bed hydrodynamics wasstudied via residence time experiments and correlated to the observed amount ofprotein captured.Experimental data indicated that optimum performance,as judged by Pe and Qdyn, was obtained when 30 column length, 1X compactiondegree, and an 210 cm/h linear flow was utilized. The novel cation exchangematerial shows promise for the direct capture and purification of a range ofbiotechnology-derived products.