Optimization of hydrodinamical and adsorptive properties of Chromatographic Gigaporose Columns.

MIRNA SANCHEZ; LEANDRO MARTINEZ; ESTEFANIA ACHILLI; MARIANO GRASSELLI; MARCELO FERNANDEZ LAHORE

Rhode Island

Conferencia; 17th International Conference on Biopartitioning and Purification 2013; 2013

Optimization ofhydrodinamical and adsorptive properties of Chromatographic Gigaporose Columns.Mirna L. Sanchez  [1,2],Leandro J. Martínez [1]EstefaniaAchilli[1] MarceloFernandez-Lahore [2] and Mariano Grasselli[1] misanchez@jacobs-university.de[1] Universidad Nacional de Quilmes- IMBICE (CONICET). [2] Jacobs University Bremen. Modernbiotechnology heavily depends on the availability of efficient processes, whichhave to generate competitive products in terms of quality and cost. Mostcommonly, the purification of bioproducts is based on chromatographicplatforms. However, the low productivity imposed by diffusional limitations,the need for multiple separation steps, and the requirement of extensive solidsseparation, drives to a technological situation characterised by low globalbioproduct yields [1]. In aprevious work, we fabricated porous, opened, and reticulated polyurethane foamssupporting a functional hydrogel layer. Preliminary studies showed that theadsorptive properties of these materials allow the reversibly binding ofproteins by ion exchange mechanisms.Dynamicbinding capacity tests (DBC) were performed employing lysozyme (HEWL) on acation exchanger matrix. DBC experiments were carried out as a function columnlength, compression degree and flow velocity. Furthermore, bed hydrodynamicswas studied via residence time experiments and correlated to the observedamount of protein captured.Experimental data indicated that optimum performance, as judged byPe and Qdyn, was obtained when 6 column length, 3X compaction degree, and an 212cm/h linear flow was utilized.The novel cation exchange material shows promise for the directcapture and purification of a range of biotechnology-derived products.