Biochemical and Functional retinal changes in a new experimental mouse model of diabetic retinopathy

MARIA CONSTANZA PAZ; PAULA SUBIRADA; MAGALI EVELIN RIDANO; CLAUDIA CASTRO; MARIA CECILIA SANCHEZ

Seattle

Congreso; Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO) 2016; 2016

The Association for Research in Vision and Ophthalmology

Purpose : Type 2 diabetes mellitus (T2DM), which is a metabolic syndrome (MS), triggers micro vascular complications in the retina know as diabetic retinopathy (DR). Several animal models of DR have been very useful; however, none of these have been able to reproduce completely anatomic and functional abnormalities of this pathology.In this work we propose to characterize a mouse model of DR that involves the SM alterations and mimics the different stages observed in humans.Methods : For this purpose, we used C57BL/6 (WT) and Apolipoprotein E knockout mice (ApoE-KO) either a normal chow (ND) or a 10% w/v fructose (FD) in drinking water from 2 months of age. At 8 months old, lipid and glucose levels in blood were analyzed and retinal function studies (ERG) were performed. Histochemical and immunofluorescence (IF) analysis were performed on retinal tissue.Results : After the 6 month feeding period, WT FD mice (n= 9) as well as ApoE-KO ND (n=10) and FD (n= 9) showed hyperglycemia and dyslipidemia. At this time, a significant decrease in the ERG b- wave and oscillatory potentials (Ops) amplitudes were observed compared to WT ND mice (p