ANTIMICROBIAL AND CELL VIABILITY MEASUREMENT OF CHITOSAN NANOPARTICLES TO BE APPLIED AS THERAPY IN BOVINE MASTITIS

ORELLANO MS; BOHL LP; ISAAC P; BRESER ML; FALCONE D; PORPORATTO C

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2016

Sociedad Argentina de Inmunología

Staphylococcus is the genus commonly isolated from bovine mastitis in many countries, which can express several virulence factors, including biofilmformation associated with persistent infections. The difficulty in eradication and the increasing concerns on antibiotics usages underscore the interest indeveloping new tools to control staphylococcal mastitis. The use of nanomaterials in medicine has enabled the development of more effective therapies.The purpose of this study was to evaluate the antimicrobial activity and anti-biofilm capacity of chitosan nanoparticles (ChNP) against bovine mastitisisolates, as well as their cytotoxic effect on bovine mammary epithelial cells (MAC-T), macrophages (BoMAC) and kidney cells (MDBK). ChNP wereobtained using reverse micelles (RM) as nanoreactors and prepared by cross-linking reaction of glutaraldehyde and native Ch inside of nheptane/sodium1,4-bis-2-ethylhexylsulfosuccinate (AOT)/water. ChNP were characterized by dynamic light scattering showed a size of 134nm. Minimuminhibitory (MIC) and bactericidal (MBC) concentrations of ChNP were determined against S. aureus (29313 and V329) and S. xylosus (1007) strains, andthe biofilm biomass and cell viability were quantified spectrophotometrically after staining with crystal violet and MTT. The results show that ChNP haslower MIC and MBC values and stronger ability to inhibit biofilm formation of the bacteria tested than Ch (200 vs 1600 µg/ml; p