Planktonic and biofilm lifestyles: setup and preliminary results of a methodology to study the interactions of microorganisms in a bovine mastitis in vitro model.

BOHL LP; LERDA WE; CONESA A; ISAAC P; BRESER ML; VALLE J; TOLOSA DE TALAMONI N; PORPORATTO C

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2016

Sociedad Argentina de Inmunología

Staphylococcus aureus (SA) is the main pathogen isolated into intramammary infections from dairy cattle. SA has the ability to grow as biolm, showing a reduction to antimicrobial susceptibil-ity and to the recognition by the immune system. However, the response of epithelial cells to planktonic and biolm cultures has not been characterized. The aim of this work was to study the immune response of bovine mammary epithelial cells to SA grown in planktonic and biolms form. The study was performed using a cell line of bovine mammary epithelial (MAC-T) infected with dif-ferent form of SA V329 grown. To determinate the interaction of biolm grown, bacteria was cultures in a specic device consisting of a lid with 96 pegs. The CFU of planktonic and biolm grown was normalized to co-cultures assays. MAC-T cells infected with planktonic and biofilms SA was evaluated to 2, 4, 6 and 24 h. Co-cultures interaction was evaluated by the internaliza-tion of SA (CFU) (2 h), pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) mRNA expression (4 h) and IL-1β and IL-6 production in the supernatants by ELISA (2, 4, 6 and 24 h). Bacteria from planktonic cultures shown higher internalization than bacteria from biofilms cultures (p