A blaVIM-2 plasmid disseminating in extensively drug-resistant clinical Pseudomonas aeruginosa and Serratia marcescens isolates

ELISABET VILACOBA; CECILIA QUIROGA; MARIANO PISTORIO; ANGELA FAMIGLIETTI; HERNAN RODRIGUEZ; JAIME KOVENSKY; MAXIME DERASPE; FREDERIC RAYMOND; PAUL H. ROY; DANIELA CENTRÓN

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

AMER SOC MICROBIOLOGY

Lugar: Washington; Año: 2014 vol. 58 p. 7017 - 7018

0066-4804

I nfections caused by carbapenem-resistant Enterobacteriaceae isolates are an issue of major global concern (1). Genes coding for metallo--lactamases (MLs) identified in clinical isolates are associated with mobile elements and subject to horizontal genetic transfer (HGT) events (2?6). VIM-2 is present on numerous plasmids, but only pNOR-2000 from Pseudomonas aeruginosa COL-1 from France (7, 8) and pLD209 from Pseudomonas putida LD209 from Argentina (9) have been completely sequenced. Here, we report the complete sequence and characterization of plasmid pDCPR1 harboring a blaVIM-2 gene cassette in a Tn402-type class 1 integron, which was isolated from two extensively drug-resistant strains: P. aeruginosa 802 (from a burn patient at the Hospital Municipal de Quemados, Argentina, 2005) and S. marcescens 68313 (Sanatorio Sagrado Corazón, Argentina, 2012).