INVESTIGADORES
RODRIGUEZ Fernanda Mariel
artículos
Título:
Influence of insulin-like growth factor-binding proteins-2 and -3 in the
Autor/es:
RODRÍGUEZ FM.; SALVETTI NR.; PANZANI CG; BARBEITO CG.; ORTEGA HH.; REY F.
Revista:
ANIMAL REPRODUCTION SCIENCE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 p. 1 - 10
ISSN:
0378-4320
Resumen:
Ovarian cysts are one of the major causes of infertility in dairy cows. The development is
associated with an endocrine imbalance in the hypothalamohypophysealgonadal axis
in which endocrine factors participate in follicular growth and differentiation and in the
secretion of ovarian hormones. Insulin-like growth factor family are essential local regulators
of ovarian follicle development and functionality and actions are mediated by binding
protein activity. The aim of the present study was to analyze the expression of IGFBP-2 and
IGFBP-3 in developing follicles of normal estrous cycling animals and with spontaneous and
induced cystic ovarian disease (COD) to determine IGF bioavailability. The mRNA of IGFBP-2
and IGFBP-3 in follicular walls was quantified by reverse transcription-polymerase chain
reaction (RT-PCR) and in situ hybridization. Protein expression was analyzed by immunohistochemistry.
The results demonstrated reduced amounts of mRNA of both IGFBPs in the
granulosa cells of ovarian follicles of animals with COD (P < 0.05). The present study suggests
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.
P < 0.05). The present study suggests
that the IGF system or imbalances between IGFs and IGFBPs may be involved in COD
of cattle.