Stable and Reusable Fe3O4/ZIF-8 Composite for Encapsulation of FDH Enzyme under Mild Conditions Applicable to CO2 Reduction

AGUIRRE, MATÍAS E.; RAMÍREZ, CRISTINA L.; DI IORIO., YESICA

CHEMISTRY-A EUROPEAN JOURNAL

WILEY-V C H VERLAG GMBH

Año: 2023

0947-6539

The enzymatic reduction of carbon dioxide presents limited applicability due to denaturation and the impossibility of biocatalyst recovery; disadvantages that can be minimized by its immobilization. Here, a recyclable bio-composed system was constructed by in-situ encapsulation under mild conditions using formate dehydrogenase in a ZIF-8 metalorganic framework (MOF) in the presence of magnetite. The partial dissolution of ZIF-8 in the enzyme´s operation medium can be relatively inhibited if the concentration of magnetic support used exceeds 10 mg mL−1. The bio-friendly environment for immobilization does not harm the integrity of the biocatalyst, and the production of formic acid is improved 3.4-fold compared to the free enzyme because the MOFs act as concentrators of the enzymatic cofactor. Furthermore, the bio-composed system retains 86 % of its activity after a long time of five cycles, thus indicating an excellent magnetic recovery and a good reusability.