GENOME DRAFT SEQUENCE OF A CELLULOLYTIC AND XYLANOLYTIC ISOLATE OF CELLULOMONAS FLAVIGENA

PICCINI F.E:; MURUA Y.; GHIO, S; LIA, V; TALIA P; RIVAROLA M; CAMPOS E

Rosario

Congreso; L Reunión Anual Sociedad Argentina de Investigación en Bioquimica y Biología Molecular- SAIB 2014; 2014

SAIB

Lignocellulose degrading bacteria have high biotechnological potential in biofuels, feed and paper industries. We have isolated a novel Cellulomonas flavigena strain, named B6, from a soil consortium. A draft version of the genome was obtained using the Illumina MiSeq sequencing platform at the UGB laboratory of INTA. The data comprised a number of 1.532.556 paired-end reads, with a length of 500 bp. It was assembled in 512 contigs and annotated by the IGS Annotation Pipeline (http://ae.igs.umaryland.edu). Different bioinformatics analysis showed a high and even coverage of the reference genome C. flavigena type strain (134T). Moreover, the ordering of these contigs with the reference genome suggests we have approximately 75% of the de novo genome in the correct ordering. C. flavigena B6 has a 74.7% GC content, which is consistent with the previous published data for C. flavigena reference strain. Also, interestingly, the initiation codon GTG is present at a high frequency, approximately 62% of start codons. The isolate secretes free cellulases and xylanases and presents mainly high xylanase activity. Coincidently a great number of beta-1,4- endoxylanases coding sequences have been identified in the genome. In summary, we have isolated a Cellulomonas flavigena strain with high potential as source of enzymes for biomass deconstruction.