Biochemical responses of the golden mussel Limnoperna fortunei under dietary glyphosate exposure

IUMMATO M.M.; SABATINI S; CACCIATORE LUIS; COCHÓN ADRIANA; CATALDO D.; DEL CARMEN RÍOS DE MOLINA, MARÍA; JUÁREZ A.B.

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2018 vol. 163 p. 69 - 75

0147-6513

The aim of this study was to analyze the biochemical alterations in the goldenmussel Limnoperna fortunei under dietary glyphosate exposure. Mussels were fedduring 4 weeks with the green algae Scenedesmus vacuolatus previously exposed toa commercial formulation of glyphosate (6 mg/ L active principle) with the addition ofalkyl aryl polyglycol ether surfactant. After 1, 7, 14, 21 and 28 days of dietaryexposure, glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase(SOD), acetylcholinesterase (AChE), carboxylesterases (CES) and alkalinephosphatase (ALP) activities, glutathione (GSH) content and damage to lipids andproteins levels were analyzed. A significant increase (72%) in the GST activity and asignificant decrease (26%) in the CES activity in the mussels fed on glyphosateexposed algae for 28 days were observed. The ALP activity was significantlyincreased at 21 and 28 days of dietary exposure (48% and 72%, respectively). GSHcontent and CAT, SOD and AchE activities did not show any differences between theexposed and non exposed bivalves. No oxidative damage to lipids and proteins,measured as TBARS and carbonyl content respectively, was observed in responseto glyphosate dietary exposure. The decrease in the CES activity and the increases in GST and ALP activities observed in L. fortunei indicate that dietary exposure toglyphosate provokes metabolic alterations, related with detoxification mechanisms.