INVESTIGADORES
SAUKA Diego Herman
artículos
Título:
Identification, cloning and expression of an insecticide cry8 gene from Bacillus thuringiensis INTA Fr7-4
Autor/es:
AMADIO AF; NAVAS LE; SAUKA DH; BERRETTA MF; BENINTENDE GB; ZANDOMENI RO
Revista:
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
KARGER
Referencias:
Lugar: Basel; Año: 2013 vol. 23 p. 401 - 409
ISSN:
1464-1801
Resumen:
Insecticidal activity of Bacillus thuringiensis is attributed
largely to the crystal proteins. These were found with specific
toxic activity against insects in different orders. A novel
cry8 gene from B. thuringiensis strain INTA Fr7-4 from Argentina
was characterized. The encoded protein, Cry8Qa2, is
1184 amino acids long and its sequence is more similar to
those of Cry8F class. We cloned and expressed the protein in
an acrystalliferous strain of B. thuringiensis using two differentially
regulated promoters. The recombinant strains produced
ovoid crystals with low toxicity against larvae of Anticarsia
gemmatalis (Lepidoptera: Noctuidae). The morphology
and insecticidal properties of these crystals resembled
those produced by the native strain INTA Fr7-4.
