Role of heme-oxygenase 1 in the cellular metabolism of prostate cancer

CASCARDO, FLORENCIA; PAEZ, ALEJANDRA; VELAZQUEZ, FLORENCIA; LAGE VICKERS, SOFIA; ANSELMINO, NICOLAS; ANTICO ARCIUCH, VALERIA; GUERON, GERALDINE; COTIGNOLA, JAVIER; VAZQUEZ, ELBA

Mar del Plata

Congreso; LXIII Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2018

Sociedad Argentina de Investigacion Clinica

Deregulation of cellular energetics has become one of the hallmarks of cancer evidenced by the numerous connections between signaling pathways that include oncoproteins and key metabolic enzymes. Heme Oxygenase-1 (HO-1) is a cellular homeostatic regulator counteracting oxidative and inflammatory damage. We previously showed that HO-1 has an antitumoral activity in prostate cancer cells. It inhibits cell proliferation, migration, tumor growth and angiogenesis. The aim of this project was to further study the role of HO-1 on the energetic metabolism of prostate cancer cells.In earlier studies, we demonstrated a significant reduction in ATP production and oxygen consumption rate in PC3 cells (derived from a metastatic prostate tumor) after treatment with hemin (inducer of HO-1 expression and activity) 80 µM for 24h. These results confirmed a negative regulation on the metabolic rate. In this work, in order to further analyze the regulation of cell metabolism by HO-1, we studied glucose uptake in PC3 cells. We found lower glucose uptake in cells treated with hemin (20.32 vs 3.52 fmol/cell/min; p