TOPICAL APPLICATION OF H4R LIGANDS PREVENTS ALVEOLAR BONE LOSS AND SALIVARY GLAND ALTERATIONS IN A RAT MODEL OF PERIODONTITIS

JUAN P PRESTIFILIPPO; JAVIER FERNÁNDEZ-SOLARI; DIEGO MARTINEL LAMAS; CLAUDIA MOHN; ELENA RIVERA; JUAN C ELVERDIN; VANINA A MEDINA

Lyon

Congreso; European Histamine Research Society 43rd Annual Meeting; 2014

Periodontitis is a chronic inflammatory disease of the supporting structures of the teeth initiated by pathogenic bacteria, which often leads to tooth loss. Its significant impact on the patient's general health and quality of life point toward the development of more effective management for this disease. Recently, we have reported that systemic administration of histamine and JNJ7777120 reduced experimental periodontitis (EP)-induced damage on submandibular gland (SMG) and periodontal bone structure. The aim of this study was to investigate the effects of the topic administration of the H4R ligands, clozapine and JNJ10191584 (JNJ101), on EP-induced histological, functional and inflammatory alterations in SMG, periodontal bone and gingiva. Bilateral EP was induced for two weeks by placing a cotton thread ligature around the neck of both first lower molars in anesthetized male rats (n=10 each group). Clozapine (0.25%) and JNJ101 (2.5%) in the form of gel were administered topically immediately after ligature placement and daily after periodontitis induction for 2 weeks. Results indicate that both compounds diminished the lingual and vestibular bone loss determined as distances between the cemento-enamel junction and the alveolar crest (0.76 ± 0.03 mm in clozapine group, P