Expression of Histamine H3 and H4 Receptors in Benign Lesions and Malignant Carcinomas of the Human Mammary Gland.

MEDINA VANINA; CROCI MAXIMO; CRESCENTI ERNESTO; BERGOC ROSA; RIVERA ELENA

Mayo 10-13, 2006, Delfos, Grecia

Congreso; European Histamine Research Society, XXXV Annual Meeting. .; 2006

Expression of Histamine H3 and H4 Receptors in Benign Lesions and Malignant Carcinomas of the Human Mammary Gland Vanina A Medina(1), Maximo Croci(2), Ernesto  JV Crescenti(2), Rosa M Bergoc(1), Elena S Rivera(1). (1) Radioisotopes Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, 1113 Buenos Aires, ARGENTINA. (2) Immunooncology Institute, 3200 Córdoba Av., 1187 Buenos Aires, ARGENTINA. We have previously reported that human mammary gland and benign lesions expressed both H1 (H1R) and H2 (H2R) receptors. On the other hand 100% and 75% of breast carcinomas presented H1R and H2R respectively; the absence of H2R seemed to be related with bad prognosis. The aim of present work was to determine the presence of H3 (H3R) and H4 receptors (H4R) in human mammary biopsies and cell lines (HBL-100 and MDA-MB-231). We also studied the expression of histidine decarboxylase (HDC), proliferating cell nuclear antigen (PCNA) and histamine (HA) content. Samples were obtained by surgery from 45-55 year-old women, 5 corresponded to benign lesions and 15 to breast carcinomas or metastasis. HA and proteins were determined by immunohistochemistry using specific antibodies. Results indicated that HDC expression was detectable in 50% of samples and no correlation was observed either with the other proteins or with tumor characteristics. All samples presented Ha and the content was higher particularly in carcinomas and metastasis. PCNA was positive in most biopsies, showing a lower or negative expression in benign lesions and a direct correlation with invasiveness and metastasis. In this regard PCNA and HA content showed the same tendency. With respect to H4R the benign lesions were negative and 50% of carcinomas showed a low positive mark, all of them corresponding to metastasis or high invasive tumors. The H3R were positive in all samples, showing a lower expression in benign lesions. This result was clearly evident in the benign tissue surrounding the carcinomas that in all cases presented a lower or negative expression compared to the carcinoma of the same patient.  This preliminary report is one of the first to describe the presence of H3R in human mammary tissue. In agreement the cell lines studied presented both H3R and H4R. remarkably their expression was higher than H1R and H2R as determined by Western blot and their presence may be related to cell proliferation.