Radioprotective potential of histamine on rat small intestine and uterus

E CARABAJAL; N MASSARI; M CROCI; D MARTINEL LAMAS; JP PRESTIFILIPPO; RM BERGOC; ES RIVERA; VA MEDINA

Congreso; European Histamine Research Society, XLI Annual Meeting; 2012

Based on our previous data on the histamine radioprotective effect on mouse small intestine, bone marrow and salivary glands in the present work we aimed to improve our knowledge about histamine radioprotective potential investigating its effect on reducing ionising radiation-induced injury on the rat small intestine and uterus. Rats were divided into 4 groups. Histamine and histamine-5Gy groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 hours before irradiation. Histamine-5Gy and untreated-5Gy groups were irradiated with a dose of whole-body Cesium-137 irradiation. Three days post irradiation animals were sacrificed and tissues were removed, fixed, stained with haematoxylin and eosin and histological characteristics were evaluated. Proliferation and apoptosis markers were studied by immunohistochemistry. Results demonstrate that histamine treatment reduced radiation-induced mucosal atrophy, oedema and vascular damage produced by ionising radiation, increasing the number of crypts per circumference (239 ± 12 vs. 160 ± 10, P