Prognostic potential of histamine H4 receptor expression in renal cancer.

NOELIA A MASSARI; MONICA TAQUEZ DELGADO; MELISA N NICOUD; S VILLALBA; MARÍA V HERRERO DUCLOUX; VANINA A MEDINA

Hannover

Congreso; European Histamine Research Society 50th Annual Meeting; 2022

Renal cell carcinoma (RCC) is one of the most prevalent urological malignancies worldwide. Clear cell RCC (ccRCC) represent about 80% of RCC subtypes and show distinct immunological features and low 5-year survival rate. The expression of histamine H4 receptor (H4R) was linked to clinicopathological features and tumor grade in different types of cancer. However, there is no evidence of the protein expression of H4R in RCC. The aim of this work is to first evaluate the H4R expression in ccRCC using The Cancer Genome Atlas (TCGA) data and correlated it with clinical attributes. To validate the transcriptomic data, we next evaluated the H4R protein expression by immunohistochemistry in 13 normal renal tissue and in 31 ccRCC samples. Mast cell count and tumor-infiltrating lymphocytes (TILs), including tumor-intraepithelial and stromal lymphocytes, were also analyzed.ccRCC exhibited increased H4R gene expression compared to normal tissue. Within tumor samples, G4 neoplasm histologic grade showed the lowest levels of H4R gene expression. A high level of H4R was significantly associated with increased disease-free survival (P=0.0466) in ccRCC patients.Likewise, the H4R protein expression levels were significantly higher in ccRCC specimens than in normal renal tissue (Mann-Whitney test, P