Histamine H4 receptor is expressed in human T-cell lymphoma and its activation induces antitumor effects

MARIANGELES CLAUZURE; MONICA TAQUEZ DELGADO; JM PHILLIP; MV REVUELTA; LEANDRO CERCHIETTI; VANINA A MEDINA

Hannover

Congreso; European Histamine Research Society 50th Annual Meeting; 2022

The discovery of the human histamine H4 receptor (H4R) has contributed to our understanding of the role of histamine in numerous physiological and pathological conditions, including tumor development and progression. The lymph nodes of patients with malignant lymphomas have shown to contain high levels of histamine, however, less is known regarding the expression and function of the H4R in T-cell lymphoma (TCL). The aims of the present work were to investigate the expression of the H4R in TCL, and to evaluate the potential antitumor effects of histamine and H4R ligands. Using RNAseq available data from different studies, we show the mRNA expression of H4R in human TCL samples. We validated the expression of H4R isoforms (mRNA and protein) in human aggressive TCL cell lines (OCI-Ly12, Karpas 299, and HuT78). The full length H4R and the two alternatively spliced isoforms were differentially expressed in the three TCL cell lines. Histamine and specific H4R agonists (VUF8430 and JNJ28610244) significantly reduced cell viability in a dose-dependent manner (P