METHODOLOGICAL APPROACHES TO INVESTIGATE THE EFFECTS OF HISTAMINE RECEPTOR TARGETING COMPOUNDS IN PRECLINICAL MODELS OF BREAST CANCER

DIEGO J MARTINEL LAMAS; MELISA N NICOUD; HELENA STERLE; GRACIELA P CRICCO; GABRIELA A MARTÍN; GRACIELA CREMASCHI; HUBERT SCHWELBERGER; ELENA S RIVERA; VANINA A MEDINA

Histamine Receptors as Drug Targets

Springer

Año: 2017; p. 353 - 387

Numerous studies demonstrated the pivotal role of histamine in breast cancer development and progression. This chapter aims to describe different preclinical breast cancer models, including in vitro soft agar clonogenic assay and in vivo chemically-induced breast tumors in rats, human triple negative breast cancer (TNBC) xenograft, and murine TNBC syngeneic graft in mice. All these models could be useful and complementary to assess the role of histamine receptor ligands in breast cancer, which could originate advances in novel therapeutics to treat this disease. This chapter also describes the gold standard radiometric techniques to evaluate the activity of histamine metabolizing enzymes in breast cancer specimens.