Radioactive treatment of a murine melanoma using a 32P-patch

MARIA SALGUIERO; NICOLAS COLLIA; MARIA ARCOS BARREIRO; VANINA MEDINA; JORGE NICOLINI; GRACIELA CREMASCHI; MARCELA ZUBILLAGA

NUCLEAR MEDICINE COMMUNICATIONS

Lippincott Williams & Wilkins

Lugar: England; Año: 2009 vol. 30 p. 706 - 712

0143-3636

Objective: to evaluate the therapeutic effects of a 32P-patch in the treatment of a murine melanoma. Materials and methods: 30 male C57BL6 mice were divided in 2 groups: treated (T) and control (C). Superficial tumors were induced in both groups by injecting B16F1 melanoma at about 105cells/mouse subcutaneously. Tumors developed 10-15 days after transplantation and the 32P-patch was applied on palpable tumors of T group. Tumor growth was followed in both groups by measuring tumor size with a caliper. After the follow-up period, animals were sacrificed and tumor samples of T and C groups were collected for histological study by preparing paraffin sections stained with hematoxilin-eosin. Results: the 32P-patch has demonstrated the absence of radioactivity leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The Tumor growth was followed in both groups by measuring tumor size with a caliper. After the follow-up period, animals were sacrificed and tumor samples of T and C groups were collected for histological study by preparing paraffin sections stained with hematoxilin-eosin. Results: the 32P-patch has demonstrated the absence of radioactivity leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The Tumor growth was followed in both groups by measuring tumor size with a caliper. After the follow-up period, animals were sacrificed and tumor samples of T and C groups were collected for histological study by preparing paraffin sections stained with hematoxilin-eosin. Results: the 32P-patch has demonstrated the absence of radioactivity leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The leakage in vitro as well as the homogeneous distribution of the radionuclide. The skin surface at application site of 32P-patch appeared hairless and erythema developed, but reversed to normal after a few days in T group. Tumor growth control was achieved in T group compared with C group, although complete remission did not take place. Conclusion: The 32P-patch tested for the treatment of a murine melanoma model demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other treatment modalities may be necessary to achieve complete regression. The demonstrated its efficacy since tumor growth was retarded after its application. Nevertheless, the adjustment of some therapeutic parameters and/or its combination with other t