Involvement of hydrogen peroxide in histamine-induced modulation of WM35

VANINA MEDINA; NOELIA MASSARI; GRACIELA P CRICCO; GABRIELA A MARTÍN; ROSA M BERGOC; ELENA S RIVERA

FREE RADICAL BIOLOGY AND MEDICINE

Elsevier Science

Año: 2009 vol. 46 p. 1510 - 1515

0891-5849

Histamine is a recognized growth factor in melanoma and exogenous histamine produces a dual effect on proliferation. We have previously reported that histamine at micromolar concentration reduces proliferation of melanoma cell lines. In order to investigate the mechanism by which histamine inhibits proliferation of WM35 human melanoma cells, we have studied the involvement of histamine in reactive oxygen species production and antioxidant enzymes regulation in these cells. Results indicate that histamine treatment (10 uM) significantly increased hydrogen peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells M) significantly increased hydrogen peroxide levels, while it slightly decreased superoxide levels associated with an enhancement of superoxide dismutase and a reduction of catalase activities. Additionally, catalase treatment reversed the inhibitory effect of histamine on proliferation and different treatments that reduce hydrogen peroxide formation increased proliferation of these cells. Furthermore, we demonstrated that the inhibition of proliferation produced by histamine was mediated at least in part by an induction of cell senescence. We conclude that hydrogen peroxide is involved in histamine-mediated modulation of proliferation in malignant melanoma cells