Effects of Oligoelements Se, Zn, and Mn plus Lachesis Muta Venom in Experimental Scleroderma

CRESCENTI EJV; MEDINA VA; SAMBUCO L; CREMASCHI G; GENARO A; CRICCO GP; MARTIN G; VALLI E; MARTINEL LAMAS D; PERAZZO JC; RIVERA ES; BERGOC RM

BIOLOGICAL TRACE ELEMENT RESEARCH

HUMANA PRESS INC

Lugar: Oregon; Año: 2014 vol. 157 p. 138 - 146

0163-4984

SCLERODERMA, SCLEROSIS OF THE SKIN, IS A SEVERE AUTOIMMUNE DISEASE REFRACTANT TO ALL KIND OF TREATMENTS. TO STUDY THE IN VIVO EFFECTS OF A COMBINATION OF THREE OLIGOELEMENTS SELENIUM (SE), ZINC (ZN), AND MANGANESE (MN) PLUS LACHESIS MUTA VENOM (O-LM) ON THE BLEOMYCIN (BLM)-INDUCED SCLERODERMA MOUSE EXPERIMENTAL MODEL. C3H MICE WERE RANDOMLY DIVIDED INTO FOUR GROUPS: CONTROL (PHOSPHATE-BUFFERED SALINE (PBS)), O-LM, BLM, AND BLM + O-LM. ALL ADMINISTRATIONS WERE PERFORMED SUBCUTANEOUSLY INTO THE BACK OF MICE. BLM WAS INJECTED 5 DAYS PER WEEK FOR THREE CONSECUTIVE WEEKS AND O-LM WAS ADMINISTERED SIMULTANEOUSLY WITH BLM FROM THE BEGINNING OF THE EXPERIMENTS AND LASTED FOR 3 WEEKS AFTER THE FINAL BLM OR PBS INJECTION (FOR O-LM AND BLM + O-LM GROUPS), WHEN ANIMALS WERE SACRIFICED AND HISTOPATHOLOGICAL, IMMUNOHISTOCHEMICAL, THIOBARBITURIC ACID REACTIVE SPECIES (TBARS) EVALUATION, AND AUTOANTIBODIES DETECTION WERE DETERMINED. O-LM SIGNIFICANTLY REDUCED BLM-INDUCED ENHANCED DERMAL THICKNESS (605 ± 47 VS. 956 ± 59 ΜM, P