Gametophyte development and conservation of Ctenitis submarginalis (Dryopteridaceae) in Buenos Aires Province, Argentina

RAMOS GIACOSA, J. P.; GORRER, D. A.; GIUDICE, G. E.; LUNA, M. L.

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

SOCIEDAD ARGENTINA DE BOTÁNICA

Lugar: Córdoba; Año: 2017 vol. 52 p. 697 - 703

0373-580X

The spore germination and gametophyte development of Ctenitis submarginalis from PuntaLara Natural Reserve, Buenos Aires province were studied as a contribution to the conservation of thespecies in this protected area. Spores collected from natural populations were sowing in Dyer mediumand germinated in six day with a percentaje of 88 % fifteen days after sowing. The germination is Vittariatype and the gametophyte development is Aspidium type. The gametophyte of Ctenitis submarginalis iscordate, with a deep notch and well developed and symmetric wings. The gametangia appear 50 daysafter sowing. The sporophyte emerges 190 days after sowing and two different hairs were observed in thefirst lamina. The in-vitro spore germination is a successful method for obtaining numerous sporophytes ofCtenitis submarginalis. The sporophytes obtained were gradually acclimated, transplanted to its naturalhabitat in the reserve and will be monitored frequently to contribute to the conservation of the species.