In vitro spore culture and reproductive aspects of the annual fern Anogramma chaerophylla (Pteridaceae)

LUNA, M. L.; YAÑEZ, A.; RAMOS GIACOSA, J. P.; GORRER, D. A.; BERRUETA, P. C.; GIUDICE, G. E.

BOLETíN DE LA SOCIEDAD ARGENTINA DE BOTáNICA

SOCIEDAD ARGENTINA DE BOTÁNICA

Lugar: Córdoba; Año: 2016 vol. 51 p. 675 - 682

0373-580X

Anogramma chaerophylla belongs to a fern genus with annual sporophytes and potentiallyperennating gametophytes. In the studied area, Natural Reserve Punta Lara, the plants grow stressedmainly by pollution and the invasion of exotic species. As a part of a project on conservation of fernsinhabiting Buenos Aires province, the objectives of this work were to evaluate in Anogramma chaerophyllathe conditions for in-vitro spore germination and to analyse the different stages of its reproductive cycle.Spores were sterilized in an aqueous solution 10 % of NaCIO during different times and then sown in Petridishes with Murashige & Skoog medium, without the addition of sucrose. The dishes were kept underlaboratory conditions, at 12 h light/ darkness photoperiod and a temperature of 22 (± 2) °C. After twoweeks, the percentage of germination was 80%. The spore germination pattern corresponds to the Vittariatype and the prothallus development was Ceratopteris type. Gametangia developed first in the bendedthalloid region of the prothallus and then bisexual tubercles originated near this zone. The sporophytesdeveloped only in association with the tubercles. During culture in plastic pots the sporophytes gave originto a second generation of prothalli. Our preliminary findings demonstrate that the in vitro culture techniqueis suitable for A. chaerophylla propagation as a strategy for ex-situ conservation.