Spore morphology and wall ultrastructure of Lycopodiaceae from northwest Argentina

RAMOS GIACOSA, J. P.; MORBELLI, M. A.; GIUDICE, G. E.; GORRER, D. A.

REVIEW OF PALAEOBOTANY AND PALYNOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 225 p. 84 - 94

0034-6667

Spores of Lycopodiaceae species growing in northwest Argentina were studied. The taxa are: subfam. Lycopodioideae: Austrolycopodium erectum, Diphasiastrum thyoides, and Lycopodium clavatum subsp. clavatum; subfam. Lycopodielloideae: Palhinhaea cernua; and subfam. Huperzioideae: Phlegmariurus mandiocanus, Phlegmariurus phylicifolius, Phlegmariurus reflexus, Phlegmariurus saururus, and Phlegmariurussotae. The study is based on herbarium material; spores were examined with light microscope (LM), scanning (SEM) and transmission electron microscope (TEM). Spores are trilete 24-44 μm in equatorial diameter and 14-35 μmin polar diameter. The exospore is 0.5-9.5 μmthick and one or two-layered, depending on the analyzed area of the spore. The ornamentation can be reticulate, rugulate, foveolate, fossulate orfoveolate?fossulate. The perispore is 30-190 nm thick, one or two-layered, and is only visible with TEM. The presence of this wall is mentioned and described for the first time in Phlegmariurus phylicifolius. In the studied species, spore ornamentation of the proximal face is different from or less complex than the distalface. The results evidence the significance of spore morphology in Lycopodiaceae systematics, particularly at subfamilial level.