INVESTIGADORES
COLMAN LERNER Alejandro Ariel
congresos y reuniones científicas
Título:
STUDYING THE CELL CYCLE AND PHEROMONE RESPONSE PATHWAY INTERACTION IN YEAST Saccharomycfes cerevisiae
Autor/es:
ALICIA GRANDE; ALTSZYLER E; BALENZUELA P; BRUNO L; LAJE R; ARIEL CHERNOMORETZ; ALEJANDRO COLMAN LERNER
Lugar:
Potrero de Funes
Reunión:
Congreso; SAIB 47th Annual Meeting Argentine Society of Biochemistry and Molecular Biology XLVII Reunión Anual Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Institución organizadora:
Society of Biochemistry and Molecular Biology XLVII Reunión Anual Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
In haploid yeast, mating pheromone triggers a fate decision: arrest
of the cell cycle in G1 and initiation of mating events. To do that, the
MAP kinase of the pheromone response pathway (PRP) Fus3
activates the cyclin dependent kinase inhibitor Far1, which binds to
and inhibits all three G1 cyclins complexes, Cdc28/Clns.
Cdc28/Cln2 activity is essential to pass the START G1 checkpoint,
while Cdc28/Cln3 is required earlier in G1 to drive the expression of
Cln2. This has two important consequences: a) inhibition of
Cdc28/Clns causes cells to arrest in G1, and b) since Cdc28/Cln2
can block pheromone response, its inhibition by Far1 acts as a
positive feedback loop. Because the signaling pathway is physically
supported by a network of interacting proteins, evolving in space
and time according to fundamental laws of reaction, diffusion and
transport, here we propose a basic mathematical model, with a small
number of parameters and dynamical variables, that reproduces the
observed biological phenomena: the interaction between the cell
cycle and the PRP. As a first approximation, we used a set of
nonlinear ordinary differential equations (ODE) that describe the
dynamics of the simultaneously occurring reactions. We tested our
model using fluorescent microscopy, measuring fluorescent
proteins used as reporters of the PRP and morphology for the cell
cycle.