INVESTIGADORES
DI VENOSA Gabriela Mariana
capítulos de libros
Título:
Studies on extracts of Argentinean plants: cytotoxic tumoral activity per se and as photosensitizers in
Autor/es:
BATLLE A; MAMONE L; CASAS A; RODRIGUEZ L; DI VENOSA G
Libro:
In the hope of going over the present Clinical PD and PDT". A Symposium Book in "Pacifichem 2010
Editorial:
Sankeisha Co., Ltd
Referencias:
Lugar: Nagoya; Año: 2011; p. 4 - 22
Resumen:
Photodynamic Therapy (PDT) and Photodiagnosis (PD)
can be used for the treatment and diagnosis of tumors and other non-tumoral diseases. PDT is based
on combining the use of a photosensitiser (PS), light and oxygen. There are several PSs, among them
there exist some natural products, obtained from plants. Our aim has been to investigate extracts, obtained
from autochthonous plants, looking for new PSs and to evaluate their cytotoxic properties per se. Next,
we plan to purify the photoactive compound, determine its chemical structure and attempt its synthesis.
Extracts from 40 plant species have been studied. Time exposure to tumour cells was 24h. It was
found that exposure of the murine tumour line LM2 to the methanol extracts of 10 ìg/ml of Combretum
fruticosum and 50 ìg/ml of Scutia buxifolia leaves and irradiation with light doses of 0.073 J/cm2 and
0.019 J/cm2, respectively, induced 50% death. Methanol extracts from 0.01 mg/ml of Iochroma australe
and 0.025 mg/ml of Jacaranda mimosifalia flowers and 0.08 mg/ml of Collaea argentina leaves as well
as aqueous extracts from 0.1 mg/ml of Solanum verbacifolium flowers, were inducing 50% death in LM2
cells. Treatment of human mammary tumour cell line HB4a-Ras with around 50 ìg/ml of Iochroma austrae
and Solanum verbacifolium flowers, also induced 50% death. Exposure of the murine tumour line LM3
to 50 ìg/ml of crude methanol extracts of Collaea argentina and Macfadyena unguis cati, induced 50%
death, irradiating with 0.43 J/cm2 for C. argentina and 0.53 J/cm2 for M. unguis cati respectively.Irradiations
have been performed using a last generation 630 nm Lumiia Laser, the first prototype developed in
Argentina. Comparative studies with this Laser and others we had been using, were done, showing that
Lumiia Laser, is very easy and friendly to use, portable, more powerful and far more economic than
other similar equipments.